DateTime.Now gives the date and time as it would appear to someone in your current locale. I'd recommend using DateTime.Now whenever you're displaying a date to a human being - that way they're comfortable with the value they see - it's something that they can easily compare to what they see on their watch or clock.
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datetime.now() returns the current time as a naive datetime object that represents time in the local timezone. That value may be ambiguous e.g., during DST transitions ("fall back").